Part:BBa_K2070004

ecf35 inducible GFP reporter

This part contains an Ecf30 promoter, RBS, GFP and double terminator. Under the presence of Ecf35 (BBa_K2070009) , the promoter can be activated to generate GFP.

Experimental Validation

Measurement of ECF sigma35 and sigma30 promoter activity. Fluorescence/OD600 of a construct containing both an ECF sigma35 generating device and ECF sigma 35 inducible GFP generating device BBa_K2070005 was compared to that containing only either one of the devices. *** for P> 0.001 indicates an extremely significant difference between the induced and uninduced state.

We have also measured the strength of the activated promoter relative to BBa_J23101.

Measurement of Activated Sigma30 Promoter Activity in Comparison to Promoter BBa_J23101. Both parts are ligated in plasmid pSB3K3. The part containing the constitutive sigma35 generator was double transformed into the cell for measurement. Data represents an average of 3 samples and error bars represent the standard deviation. The promoter in its activated state is measured to have a RPU of 3.2 units.